Open Access Public Health and Health Administration Review

Pakistan’s Combating Wild Poliovirus (WPV): Efforts and Lessons Learned

Dr. Bahadar Shah — 2025-06-30

Pakistan stands at a critical juncture in the history of public health. As one of only two remaining countries endemic for wild poliovirus (WPV), alongside Afghanistan, the nation carries a significant burden—and an even greater responsibility—in the global quest for eradication. The fight against polio in Pakistan is a complex tapestry woven with threads of remarkable resilience, innovative strategies, hard-won successes, and persistent, daunting challenges. From a public health perspective, understanding this landscape is paramount, not just for Pakistan but for the health security of the world. Despite decades of effort, WPV transmission stubbornly lingers, primarily in specific high-risk zones of Khyber Pakhtunkhwa (KP) and parts of Balochistan and Sindh. The year 2023 saw only 6 reported WPV1 cases – a significant reduction from peaks exceeding 300 annually, demonstrating progress. However, the continued detection of the virus in environmental samples from major cities like Karachi, Peshawar, Quetta, and Bannu underscores the silent circulation and the ever-present risk of resurgence. Key reservoirs remain in areas characterized by insecurity, population mobility, low routine immunization coverage, and persistent community refusals. Pakistan has come too far and invested too much to falter now. The recent low case numbers are encouraging but fragile. The virus exploits any gap with ruthless efficiency. The strategies are proven; the successes demonstrate what is possible. The end of polio in Pakistan is within sight. Achieving it will be one of public health's greatest triumphs, a testament to human perseverance and collaboration. It is not just an option; it is an ethical and practical imperative for the health of Pakistan's children and the world. The time for the final, decisive push is now.

An Empirical Study of the Prevalence of Free-living Protozoans from the River Indus, Sindh, Pakistan

Dr. Asma Kanwal — 2025-06-30

Protozoa are unicellular eukaryotic organisms that exist in biomes, both land and water. Out of about 200,000 currently known species of protists, over 199,000 (or about 99 %) are autotrophs or heterotrophs that occupy free-living niches, while the rest, only 1%, are obligate symbionts that live inside or on a definite host organism. The research aimed to conduct an empirical study of the prevalence of free-living protozoans from the River Indus, Sindh, Pakistan. In the case of a research study on the water quality of rivers in Hyderabad, Sindh, Pakistan, the polypropylene water bottles and plastic zip-lock bags were used to fill the samples of river water in the River Indus. To ensure the understanding of measurements of dissolved oxygen, free CO₂, phosphate, nitrate, and biological oxygen demand, water samples were put through several standard analytical processes. Results indicate that Amoeba proteus exists at high levels in every season, particularly in winter and summer. The Paramecium was most abundant during the summer. In contrast, the number of Tintinnopsis became moderate all year, but they increased a bit during the monsoon. The winter and summer months saw the lowest numbers for Oxytricha, possibly because it isn’t as adaptable to its surroundings. This virus remains stable most of the year, except for a small increase in the summer. These results highlight the usefulness of protozoan surveillance as a bioindicator in measuring the level of water quality, as well as give credence to the notion that there should always be periodic surveillance and analysis in determining policies guiding the preservation efforts.

Using AI to Integrate Multiple Omics to Predict the Mechanisms of Rare Diseases

Mukarram Sharif — 2025-07-09

Rare diseases, which affect less than 1 in 2,000 people, are difficult to diagnose and treat due to their complex pathophysiology and genetic variation. Recent advances in artificial intelligence (AI) and multi-omics technologies, including proteomics, metabolomics, and genomics, may help us better understand the molecular mechanisms underlying these diseases. This study was aimed to explore how AI integrates multi-omics data to identify biomarkers, forecast treatment targets for rare diseases, and uncover disease-causing pathways. It highlights how AI may help with data complexity, enable personalized care, and enhance predictive modeling. Despite progress, problems like consistency, model interpretability, and data scarcity persist. By integrating recent research, this paper proposes future directions to accelerate clinical translation and highlights AI-driven multi-omics as a groundbreaking approach to understanding the mechanisms underlying uncommon diseases.

Ecological and Morphological Studies of Indian Peafowl (Pavo Cristatus) of Khar Centre, Khar Division at Khirthar National Park, Sindh, Pakistan

Mohsin Ali Brohi — 2025-07-10

In Pakistan, the blue peafowl is found in Narowal and Tharparkar, and is breeding in the natural habitat of Khar Centre, Kirthar National Park, which is the only national park in Sindh. The present research on Ecological and Morphological study is carried out in an 8 km radius of Khar Centre, Kirthar National Park, from July 2021 to June 2022. The estimated population of Indian peafowls was recorded as 250. Among them, about 30%. subadult 36% and 34% peachicks, male, female ratio is 34% to 66%. Thirty specimens of Indian peafowls were collected to measure their body parameters. Average body length from beak to cloaca was measured as 64cm and 52.8cm for adult males and females, respectively. While for sub-adults, males and females, it was 49.2cm and 44cm, respectively. While for peachicks, it was 38.4 cm and 29.6 cm, respectively. Average beak length was measured as 2.96 cm to 1.5 cm in adult, subadult, and peahen. Tail length varied from 146.8 cm to 29.2 cm in different categories. Wingspan length varied from 156 cm to 54 cm, while crest length varied from 8.2 cm to 4 cm in adults and in peahens, respectively. The temperature varies from 10°C to 45°C in the area. The water used by the Indian peafowl (Pavo cristatus) has a pH. Salinity, conductivity, and TDS are (pH: 8.60±0.59), (salinity: 60±0.28 ppt), (conductivity: 1609.33±300.57 μs/cm), and (TDS: 790.67±147.64 mg/l). In this study, the estimated population of Indian peafowl (Pavo cristatus) was observed as 31 individuals/km. The results indicate that the Khar Centre in Kirthar National Park is a suitable place for the breeding and conservation of Indian peafowl. This study is the first of its kind in Khar Centre, Kirthar National Park, Sindh.

Evaluation of Response Variation of Some Species of Mosquitoes to Various Plant Extracts

Sobia Rani — 2025-07-17

This study aimed to discover the cheapest and non-toxic method to reduce the mosquito population at the larval stage. Several plant extracts were reported as biologically active against insects and pests. For a Larvicidal activity of some plant extracts, leaves of Eucalyptus Lanceolatus, Olea Ferruginea, Melia Azedarach, & Pinus Roxburghii were evaluated against the third and fourth instar larvae of Culex quinquefasciatus and Aedes aegypti by preparing various concentrations of aqueous extract of leaves from selected plants. The concentration of these plants had an ethanolic extraction on Culex quinquefasciatus and Aedes aegypti was 500ppm, 1000ppm, 2000ppm, 3000ppm, 4000ppm, and 5000ppm concentration extract, after 24 hours, ten larvae (III and IV instar stage) were placed in each polyethylene plastic container with test solutions (100ml) at a room temperature of 25 to 30 °C. It has been observed that after 24 hours the percentage of mortality rate against 5000ppm, 4000ppm, 3000ppm, 2000ppm 1000ppm and 500ppm ethanolic extract concentration were recorded for Pinus Roxburghii extract against Culex quinquefasciatus were found the best, it was observed for Larvicidal activity after 24 hours for 5000ppm is 100%, and for 4000ppm the Larvicidal activity were also noted as 100%, Among all the tested plant extracts, the Pinus Roxburghii extract against Culex quinquefasciatus for 5000ppm and 4000ppm were also found the best as the mortality rate is 100% as well as the Eucalyptus Lanceolatus extract against Aedes aegypti were also found the best as the Larvicidal activity has been observed after 24 hours for 5000ppm is 100%, and for 4000ppm, the Larvicidal activity were also recorded as 100%, The mortality depends on time of exposure, plant species and chemical composition.

Epidemiological Analysis of Theileriosis in Goats and Its Effect on Hematological Parameters in Southern Districts of Khyber Pakhtunkhwa, Pakistan

Asjad Umair Shah — 2025-07-19

Theileriosis is among the emerging issues of the wild ungulates, especially in the tropical and sub-tropical parts of the globe. The study carried out was aimed at looking into the goats having the presence of Theleria infection by identifying the presence of the infection with the use of the microscope and to see the disease impact on the hematological points. 300 blood samples from asymptomatic goats were to be taken using a convenient sampling technique at random. The simple microscopic examination was done in the Clinical Medicine and Parasitology laboratory on all the blood samples. This has seen the percentage of positive outcomes being found 34 % in the three hundred total samples. The highest prevalence was recorded in female goats (55.88%) than male (44.1%) and statistically significant (p<.04) / non-significant (P< .002) difference was observed Area wise study was also observed where the highest prevalence was recorded in the District of Karak (25.49%), followed by Bannu (20.58%), Lakki Marwat (16.66%), DIKhan (13.72%), while the lowest prevalence was recorded in the District of Tank (11.76%). Statistically, a significant (p<.04) / (P< .002) non-significant difference was observed, where the p value (p<.0000). The alterations in hematological parameters were also observed, where the highest increase was observed in the District Karak (KC) 26/103×100. while the number of DIKhan (DIK) 11/103×100 was seriously decreased.

Global Strategies for Combating Antimicrobial Resistance through the One Health Approach: A Mini Review

Haleema Sadia — 2025-07-27

The introduction of antibiotics revolutionized medicine by significantly reducing the burden of bacterial infections. However, antimicrobial resistance (AMR) has emerged as a major global health and economic challenge. AMR arises primarily from the misuse and overuse of antimicrobials in human health, agriculture, and animal husbandry. It leads to treatment failures, prolonged illnesses, and increased mortality. The One Health approach, which integrates human, animal, and environmental health, provides a comprehensive framework for addressing AMR. The extensive use of critically important antimicrobials such as fluoroquinolones, cephalosporins, colistin, tetracyclines, and macrolides in food-producing animals is a key driver of resistance. Prudent antimicrobial use, infection prevention, and enhanced hygiene are vital in human healthcare. In animal agriculture, reducing prophylactic and growth-promoting antimicrobial use is essential. Surveillance systems help track resistance trends across sectors. Internationally, the WHO-led Global Action Plan (GAP), supported by the FAO and WOAH, emphasizes antimicrobial stewardship, resistance surveillance, and policy harmonization. At the national level, countries implement National Action Plans (NAPs) that promote public awareness, antimicrobial regulation, and research into alternatives. Tackling AMR requires a coordinated multisectoral response. The One Health approach embedded in both national and global strategies is crucial for preserving the efficacy of antimicrobials and safeguarding health across species and environments.

Genomic Analysis of Cephalosporin Resistance in Salmonella: A One Health Perspective on the Transmission of Resistance Genes between Animals, Humans, and the Environment in Punjab, Pakistan

Mahnoor — 2025-07-27

Antimicrobial resistance (AMR) in Salmonella, particularly to third-generation cephalosporins, is a rising public health threat, especially in developing regions with dense human-animal-environment interactions. This study evaluates cephalosporin resistance in Salmonella through a One Health lens in Punjab, Pakistan, using whole-genome sequencing (WGS) to uncover resistance mechanisms and transmission dynamics. Phenotypic resistance was determined through antimicrobial susceptibility testing, while genotypic resistance was characterized using WGS. Phylogenetic analysis and multilocus sequence typing (MLST) were used to map potential transmission routes. Salmonella was isolated from 33.4% of samples, with the highest prevalence in poultry (42%). Cephalosporin resistance was phenotypically observed in 52.7% of isolates, with 72.7% being ESBL-producers. WGS revealed the presence of CTX-M-15 (58%), TEM-1 (29.5%), and CMY-2 (20.5%) among resistant strains. Significant SNP-based phylogenetic relatedness was observed between human, animal, and environmental isolates, indicating active cross-sector transmission. Notably, poultry and wastewater sources were major transmission hubs. This study presents robust genomic evidence that cephalosporin-resistant Salmonella is circulating across animal, human, and environmental sectors in Punjab, Pakistan, with poultry acting as a key reservoir. The detection of widespread resistance genes such as CTX-M-15, TEM-1, and CMY-2, along with evidence of mobile genetic elements (e.g., IncFII, IncI2 plasmids), underscores the critical role of horizontal gene transfer in resistance propagation. The strong genetic relatedness between isolates from live bird markets, hospitals, and wastewater suggests ongoing transmission across the One Health spectrum.

Assessing the Impact of Internal, External, and Demographic Factors on Bank Performance: A Correlation Analysis

Mahfooz Ali Kiany — 2025-08-05

A thorough quantitative correlation analysis of the internal (capital adequacy, liquidity risk, asset quality, and employee skills), external (consumer behavior, market competition, and economic conditions), and demographic (experience, age, gender, and education) factors affecting bank performance is carried out in this study. Pearson correlation coefficients are used in the study, which applies meta-analytic techniques to 127 empirical publications (2020–2024). Key findings show strong relationships: employee training improves customer satisfaction (r = 0.38, p < 0.01), liquidity risk has a negative influence on NIM (r = -0.35, p < 0.05), and capital adequacy positively correlates with ROA (r = 0.42, p < 0.01). Demographic considerations account for 19% of the adoption of digital transformation, while economic conditions enhance capital-performance links by 28%. The most important element is asset quality (0.62*** on Z-score), but liquidity risk is a serious concern (-0.47***). One of the limitations is the possibility of missing variables. To maximize stability and profitability, banks should give priority to asset quality, liquidity management, and staff training, according to practical consequences. The report offers bankers and regulators empirically supported insights to improve performance tactics.

The Philosophical Basis of Nursing: Understanding Theory, Education, Ethics, and Clinical Practice

Roheeda Amanullah — 2025-08-18

The purpose of this study is to investigate the philosophical underpinnings of nursing by looking at the intersections of theory, education, ethics, and practice. Its objectives are to: (1) examine the role of philosophy in nursing knowledge; (2) evaluate the influence of theoretical models; (3) assess the influence of education; and (4) discuss ethical imperatives in care. The study employed CINAHL, PubMed, and PhilPapers to conduct a literature review, utilizing keywords such as "nursing philosophy" and "ethical theory. "Findings: Ontology, ethics, and epistemology are all integrated in nursing. Critical thinking is fostered by education, while theories (such as those of Nightingale and Watson) direct practice. Patient-centered care is based on the principles of autonomy, beneficence, and fairness. In conclusion, nursing is a profession that is both morally and intellectually demanding. Reflective, moral activity is strengthened by philosophy, which provides practitioners, educators, and students with new perspectives.

Comparison of Relative Fecundity and Early Survival of Ctenopharyngodon idella and Hypophthalmichthys molitrix through Induced Breeding

Rozina Gulab — 2025-08-20

Induced breeding is also a fundamental capability that ensures aquaculture has a visibly reliable source of seed of aquaculturally valuable species. The relative fecundity and early post-hatch survival of two cyprinid species, the grass carp (Ctenopharyngodon idella) and the silver carp (Hypophtalmichthys molitrix) were the objective of the present study. Ovaprim was injected into the brood fish at three levels of dosage, such as high, standard (0.5 ml/kg in females; 0.3 ml/kg in males), and low. Spawning behavior was noted eight hours after the injection, and the equivalent ovulation, fertilization, and hatching rates were documented systematically. At the same time, the parameters of water quality were measured during the experiment. The variation in ovulation and fertilization was sharp between the subjects who received the low dose and those who received the standard dose; however, the variation between the high dose and the standard dose was not significant. The grass carp under study in the current study hatched four times on average, with a value of 60%. Silver carp hatches, by contrast, have been unsuccessful because temperature inconsistent with both pond and hatchery waters has led to development being halted. These results show that the Grass carp is more resilient to thermal stress in comparison to the silver carp in a similar environment. Studies done in hatchery evidenced that induced breeding of grass carp resulted in good production, but reproduction of silver carp was still impaired by variable thermal regimes. These findings support the need to maintain ideal and consistent environmental settings to increase breeding success of induced aquaculture.

Ichthyofaunal Diversity at The Junction Between River Swat and River Panjkora in Bosaq: A Health Perspective

Muhammad Wajid — 2025-08-20

This study was conducted at the Government Degree College in Batkhela, District Malakand, focused on exploring and documenting the fish species in the Swat River, a crucial waterway in the Khyber Pakhtunkhwa region of Pakistan.

Six different fish species were collected from the Swat River, representing families such as Channidae, Cyprinidae, and Bagridae, including Channa punctata, Schizothorax spp, Cirhinus molitorella, Eurasian Carp (Cyprinus carpio) and Rita rita. These species were preserved using freezing and drying methods to ensure the integrity of the samples. Identification was carried out with the aid of scientific literature and expert guidance, ensuring accurate classification. The results identified six distinct species, reflecting the river's rich biodiversity and providing a baseline for future ecological monitoring. For instance, Channa punctata demonstrated adaptability in its diet and habitat, while Schizothorax spp highlighted the river's ability to support highland fish species. This study represents a comprehensive effort to document and understand the fish species of the Swat River, emphasizing the river’s ecological importance and the need for ongoing conservation efforts. The methodologies employed, from collection to identification, were rigorous and thorough, ensuring that the findings are both accurate and meaningful for future research and environmental management.

Job Performance Reimagined: Insights from the Job Characteristics Model and the Power of Fairness, Engagement, and Emotional Intelligence

Dr. Mahfooz Ali Kiany — 2025-08-21

With emotional intelligence acting as a mediator, this quantitative study of 320 telecom workers in Pakistan looks at how job qualities affect engagement and fairness perceptions, which in turn affect performance. By improving fairness and engagement, job design features like skill variation and feedback have been shown to improve performance, according to structural equation modeling. This mediated association was reinforced by emotional intelligence. The results highlight the importance of creating richer employment to enhance performance via psychological processes.

Biodiversity and Length-Weight Relationship of Rohu (Labeo rohita) and Silver Carp (Hypophthalmichthys molitrix) in the River Indus, Pakistan: A Healthcare Context

Muhammad Ahtisham Ul Haq — 2025-08-24

The primary aims of this study are to assess the biodiversity and establish length-weight relationships for two economically important fish species, namely silver carp (Hypophthalmichthys molitrix) and Rohu (Labeo rohita), in the Dera Ismail Khan region of Pakistan. Throughout one year, a comprehensive sampling approach was employed to collect data on fish populations inhabiting the River Indus and its adjacent aquatic ecosystems. To get comprehensive data on the distribution and population size of the species, a combination of traditional fishing equipment, such as gill nets and cast nets, and non-intrusive methods of data collection was employed. The findings of this study indicate that the River Indus exhibits a notable level of biodiversity, with the silver carp and Rohu species emerging as prominent members of the fish community. The development of length-weight relationships for both species, utilizing a range of size classes, will prove valuable in monitoring their growth and assessing their overall well-being within the ecosystem. The comprehension of the fish diversity and population dynamics of Rohu and Silver Carp has significant importance in Dera Ismail Khan. Moreover, the study's findings about the relationships between length and weight can serve as a valuable resource for conservation and fisheries management efforts. This, in turn, can contribute to the promotion of sustainable resource utilization and the preservation of the invaluable aquatic ecosystem under consideration.

Comment on the Frontal QRS-T angle as an Early Predictor of Mortality in Sepsis

Okkes Zortuk — 2025-09-13

Sepsis remains a leading cause of global mortality, driven by dysregulated host responses to infection, leading to multi-organ dysfunction, including cardiac impairment. There is a critical need for simple, rapid, and accessible prognostic tools. The frontal QRS-T angle (fQRSTa), a spatial vectorcardiographic marker of ventricular repolarization heterogeneity, has emerged as a powerful independent predictor of adverse cardiac events and mortality in general and cardiac populations. This short communication aims to review and synthesize recent literature on the utility of the frontal QRS-T angle as an early predictor of mortality in patients with sepsis. A narrative review of recent literature (primarily from 2015 onwards) was conducted, focusing on studies investigating electrocardiographic (ECG) predictors, cardiac dysfunction, and outcomes in sepsis, using databases such as PubMed and Scopus. Emerging evidence indicates that a wide fQRSTa (>90°-100°) is significantly associated with increased short-term and long-term mortality in septic patients. This electrical biomarker likely reflects the underlying septic cardiomyopathy, characterized by global myocardial depression, ischemia, and electrophysiological instability induced by the systemic inflammatory response. The frontal QRS-T angle is a promising, readily obtainable, and low-cost ECG parameter that serves as an early marker of mortality risk in sepsis. Its integration into initial risk stratification could help identify high-risk patients who may benefit from more aggressive hemodynamic monitoring and management. Future prospective, multi-center studies are needed to validate standardized cut-off values and establish their definitive role in clinical sepsis algorithms.

Service Quality and Cost of Care as Preferred Strategies for Patient Satisfaction and Loyalty: The Mediating Role of Brand Image and Perceived Value in Healthcare Services

Muhammad Siddique Khan — 2025-09-17

Satisfying patients and gaining their loyalty has equal importance in a healthcare setup as that of the clinical outcome. However, patient satisfaction is closely associated with service quality, and financial considerations have gained prominence in defining the perceived value and value creation not only in strategy literature but also in healthcare. This study explores the effects of service quality and cost of care on patient satisfaction in defining the perceived value and brand image and creating loyalty in Pakistan’s hospital sector, as both the direct association and mediators. A non-probability convenience sampling method was used, with a sample size of 180 patients, using a cross-sectional, quantitative design in private and public hospitals in Karachi City. The findings reveal that the cost of care significantly influences patient satisfaction and perceived value, both of which improve patient loyalty. While service quality positively shapes brand image, its influence was not found to be significant, yet it contributes to loyalty. These results reflect the critical role of affordability and perceived value in shaping patient experiences. The study highlights that healthcare organizations must balance quality with cost efficiency by ensuring transparent pricing and effective service delivery. Moreover, the integration of patient-centered strategies and digital health solutions can further reinforce satisfaction and loyalty, enabling hospitals to develop a sustainable pathway to improving patient relationships and competitive positioning.

Tackling the Threat: A Review of Fluoroquinolone Resistance in Salmonella and Campylobacter in Food-Producing Animals

Adeena Gul Malik — 2025-09-19

This paper explores the serious public health threat posed by fluoroquinolone resistance in Salmonella and Campylobacter bacteria, which are major causes of foodborne infections. The widespread use of fluoroquinolone antibiotics in food-producing animals has led to an increase in antimicrobial resistance (AMR), significantly limiting treatment options for human infections. This review examines the causes, prevalence, and transmission of fluoroquinolone resistance through a comprehensive literature review. Resistance is primarily driven by chromosomal mutations in genes like gyrA and parC, and the acquisition of plasmid-mediated resistance (PMQR) genes such as qnr. The prevalence of resistance is particularly high in poultry from countries like South Korea and China, with documented high rates of resistance to ciprofloxacin and nalidixic acid. Resistant bacteria are transmitted to humans through direct contact with animals and the food chain. The overuse of antibiotics in animal husbandry, horizontal gene transfer, and poor farm hygiene creates a selective pressure for resistance. The review highlights the need for more standardized and integrated surveillance programs to track resistant strains effectively. A "One Health" approach is crucial, involving stronger regulations, improved biosecurity, and the exploration of alternatives to antibiotics, such as vaccines and bacteriophages. This collaborative strategy is essential to safeguard public health against this global threat.

Acne Vulgaris among Youth: Its Prevalence, Knowledge, and Lifestyle Association

Dr. Noor Ul Huda — 2025-09-21

Acne vulgaris, a common skin condition, causes social anxiety and emotional distress, particularly among young people aged 15-30. With its increasing global prevalence, it's crucial to educate the public, especially youth, about this disease. This study aimed to assess the basic knowledge of acne vulgaris among young adults and to review its prevalence and association with lifestyle factors. The research involved an observational study and an 18-question survey, which covered participants' demographics, their understanding of acne's causes and effects, and their knowledge of its treatments and management. Conducted among 182 medical students, the study found that while 59.1% had a good understanding of the disease and its causes, a significant portion (37.6%) was poorly informed about its prevalence and effects. The results highlight a continued lack of awareness about acne and the potential complications of untreated cases, indicating a need for greater community education. This narrative review aims to provide a quick and easy-to-understand resource to help individuals better grasp the fundamentals of acne vulgaris and its outcomes, thereby enhancing awareness within the community.

Ichthyofaunal Diversity of Baran Dam District Bannu, Khyber Pakhtunkhwa, Pakistan: One Health Perspective Analysis

Muhammad Yasir Khan — 2025-09-28

Fish are cold-blooded vertebrates adapted to life in water. They breathe through gills and have a pair of fin-like appendages if present. Fish are among the most successful biodiversity of vertebrates in that they can exist in almost every type of aquatic habitat, including glacial water and new springs, as well as it may also endure an extremely wide range of salinity. The objective of the current research was to determine the ichthyofaunal richness and the place that fishes play in the Baran Dam, Bannu ecosystem, Khyber Pakhtunkhwa, Pakistan. The research work was conducted from a public health perspective from May 2023 to May 2024. In this study, 10 species were reported, namely Notopterus notopterus, Oreochromis mossabicus, Mystus bleekeri, Mystus seenghala, Ompok pabda, Ompok bimaculatus, Cirrhinus mrigala, Cyprinus carpio, Pampus argentus, and Channa punctatus. Famiy notopteridae was represented by Notopterus notopterus, and family cichlidae was represented by Oreochromis mossabicus. Mystus bleekeri, Mystus seenghala, Ompok pabda, and Ompok bimaculatus were represented by the family Siluridae; Cirrhinus mrigala and Cyprinus carpio were represented by the family Cyprinidae. Family Bramidae and family Channidae were represented each by a single species, Pampus argentus and Channa punctatus, respectively. The present study reveals that the environmental conditions of Baran Dam, Bannu, are favorable for fish survival and growth. Hence, it helps in producing healthy fish, and they are not injurious to human health. The results of this research have important implications for important conservation efforts as well as nutrient-rich food for the people of the area of Baran Dam, Bannu, Khyber Pakhtunkhwa, Pakistan.

Comparative Evaluation of Plant-Protein Blends and Mustard Oil as Dietary Regimes on Growth Performance and Whole-Body Proximate Composition of Major Carps

Syed Rahmanullah Shah — 2025-10-06

This study evaluated the proximate composition of Cyprinus carpio, Labeo rohita, and Hypophthalmichthys molitrix fed on plant-based diets compared with controls, along with the nutrient profile of the plant feed. A comparative experimental design was used to assess nutrient variations among fish species and diets. The experiment was conducted at Ratta Kulachi Hatchery and related laboratory facilities. Fingerlings of three species were divided into control and plant-fed groups. Moisture, ash, fiber, crude fat, crude protein, nitrogen-free extract (NFE) and energy were determined by standard methods. Plant feeding significantly alters nutrient composition across species. In C. carpio, plant-fed fish showed higher moisture (72.51%) but reduced fat (4.0%) compared to control (70.32% moisture, 5.0% fat). L. rohita on plant diet had higher protein (13.0%) but lower fat (3.90%) versus control (12.22% protein, 6.0% fat). Plant-fed H. molitrix showed the highest protein (16.0%) and fiber (3.33%), while its control had the lowest protein (10.0%). Ash was highest in H. molitrix control (4.0%) and lowest in L. rohita control (1.41%). NFE peaked in plant feed (65.34%) but was lowest in L. rohita control (6.01%). Energy ranged from 298.54 Kcal/100 g in plant feed to 114.40 Kcal/100 g in H. molitrix control. Plant-based diets influenced nutrient deposition differently among species, with H. molitrix showing superior protein utilization. Plant feed, rich in carbohydrates and energy, has potential as an aquaculture feed ingredient.